Leírás és Paraméterek
EDF-5.4 is an autoclavable glass bioreactor with working volume till 4,5l (total volume 6,2 l, inside diameter 150 mm).
EDF-5.4_2 for the cultivation of mammalian and plant cells.
EDF-5.4 has a compact, ergonomic and robust design. Glass cylinder vessel is mounted between the upper lid and the metallic jacketed bottom. The design of the bioreactor is easy to maintain and apply basic operations and preparatory process, in particular,
washing and autoclaving.
Extension and option possibilities
Gas mixing using mass flow controllers Hamilton ARC pH and DO sensors. These sensors send 4-20 mA signals directly to PLC and ensure at the same time information management via Bluetooth in smartphones or PC installed programs. This program generates a report about all calibration procedures, sterilization numbers, predicted service life.
Connection of additional sensors: culture turbidity (optical density), culture permittivity (viable cell density), conductivity, methanol/ethanol, off-gas analysis (O2, CO2, CH4), etc.
On-line/off-line data analysis and advanced process control possibilities using PC implemented program (Matlab, Python) algorithms communicating to process control system (PLC and SCADA).
Model
EDF-5.4_2 for cell cultures
Vessel
Total volume (L) 6.2
Working volume (L) 2 – 4,5
Inside diameter/Inside height (mm) 150/350
Aeration, mixing
Controls - Rotameter, TMFC (optional)
Aeration: EDF-5.4_2 Air / CO2, + O2, N2 (optional) Air / CO2 in excess space
Range, l / min: EDF-5.4_2 0.1 – 2 (other options available)
Bubbler: EDF-5.4_2 Microsparger
Filters: D51 mm 0.2 μm PTFE
Off-gas condenser: Water cooling
Drive unit type: Top placed magnetic drive
Mixer rotation speed (rpm): 40 - 1300
Impellers: EDF-5.4_2 Stirrers with inclined "propeller" blades
Control:Siemens Simatic S7-1500
Control Panel: Touch Screen Weintek eM3070, 5,7” TFT
Temperature: Control limits: from 5°C above cooling water up to 70 °C Measurement accuracy +/- 0.1 °C and control accuracy +/- 0.2 °C
pH: Hamilton or Mettler Toledo sensor. Acid/Alkali 0-14 +/- 0.01 pH units
pO2: EDF-5.4_2 Hamilton or Mettler Toledo sensor. Stir control + gas mixtures (optional).
Foam: Conductivity sensor
Feeding: 0.05-50 ml/min according to the set profile
Peristaltic pumps: 4 built-in variable speed pumps. Additional pumps (optional)
Dimensions: Overall dimensions of the bioreactor (mm) 930(W) х 800(H) х 600(D)