Leírás és Paraméterek
EDF-15.1 is autoclavable glass bioreactor with a working volume of 11 liters (total volume of 16 liters, the internal diameter of 200 mm).
Bioreactor is foreseen for culturing bacteria, yeast, and mycelia microorganism cultures.
However bioreactor EDF-15.1 can be adapted for culturing mammalian, stem and plant cells. Depending on the applied microbial culture it is possible to adapt the configuration of the bioreactor in a corresponding way by replacing stirrer and a bubbling system complementing aeration gas mixing unit (air, O2, N2, CO2).
EDF-15.1 has a compact and ergonomic design. Glass cylinder alias vessel is mounted between the upper lid and the metal bottom. The bottom of the vessel has a jacket for temperature control of the vessel. It also has an autoclavable sampler for aseptic sampling. The material of the vessel, which is in contact with the product, is Pyrex borosilicate glass and stainless steel 316L. Bioreactor vessel mounted on a stainless steel legs. The lid has a convenient carrying handle. The design of the bioreactor is easy to maintain and apply basic operations and preparatory process, in particular, washing and autoclaving.
Extension and option possibilities
Gas mixing using mass flow controllers Hamilton ARC pH and DO sensors. These sensors send 4-20 mA signals directly to PLC and ensure at the same time information management via Bluetooth in smartphones or PC installed programs. This program generates a report about all calibration procedures, sterilization numbers, predicted service life.
Connection of additional sensors: culture turbidity (optical density), culture permittivity (viable cell density), conductivity, methanol/ethanol, off-gas analysis (O2, CO2, CH4), etc.
On-line/off-line data analysis and advanced process control possibilities using PC implemented program (Matlab, Python) algorithms communicating to process control system (PLC and SCADA).
Total volume (L): 16
Working volume (L): 5-11
Inside diameter/Inside height (mm): 200/500
Aeration, mixing:Controls - Rotameter, TMFC (optional)
Aeration:Supply and mixing two gases (air, O2 or air, CO2) to ensure oxygen or CO2 enrichment in headspace, including 2 pressure reducer with pressure gauges; 2 rotameters (for air 0 – 20 l/min for O2 0 – 10 l/min); microbiological filter; ring sparger (optionally microsparger); inlet gas filter; outlet gas filter with easy bending condenser; membrane valve and filter
Separate gas supply or gas mixture to the surface of the culture
MFC for air, O2 or CO2
Gas mixing (air, N2, O2, CO2)
Bubbler: Ring Bubbler or Microsparger
Filters: D51 mm 0.2 μm PTFE
Off-gas condenser: Water cooling
Drive unit type: Top placed magnetic drive
Mixer rotation speed (rpm): 40 – 700
Impellers: Two standard Rushton turbines or Stirrers with inclined "propeller" blades
Control: Siemens Simatic S7-1500
Control Panel: Touch Screen Weintek eM3070, 5,7” TFT
Temperature: Control limits: from 5°C above cooling water up to 70 °C Measurement accuracy +/- 0.1 °C and control accuracy +/- 0.2 °C
Built-in thermostat (heater – 500 W, chamber, recirculation pump, cooling water valve). Temperature control range from 5 °C till 55 °C above the chiller temperature. Measurement accuracy ± 0,1 °C; accuracy of temperature within the prescribed limits ± 0,2 °C
Ports: For mixer/agitator drive; for pH, pO2, T, foam and level sensors; port with three needles for introducing additives to control the pH and foam level; hemostat tube to collect the product; aseptic pierceable membrane port, a port for the incoming gas with a filter of 0.2 micron, the condenser exhaust gas with a filter of 0.2 um, 2 spare ports closed with plugs
pH: Hamilton or Mettler Toledo sensor. Acid/Alkali 2-12 +/- 0.01 pH units
pO2: Hamilton or Mettler Toledo sensor. Stir control + О2 (optional). 0-150% +/- 1% or Hamilton or Mettler Toledo sensor. Stir control + gas mixtures (optional).
Foam: Conductivity sensor
Feeding: 0.05-50 ml/min according to the set profile
Peristaltic pumps: 4 freely configurable built-in pump with digital control (ON / OFF), and an analog supply pump with a constant flow rate of the solution (option)
Dimensions: Overall dimensions of the bioreactor (mm) 1000 (W) х 970 (H) х 600 (D)
The minimum size of the autoclave chamber for vessel autoclaving (mm)
760 (Height) х 320 (Diameter)